Eco-bioremediation potential of mealworm gut microbiome for polystyrene degradation in nutrient-based media
DOI:
https://doi.org/10.36782/apjsafe.v13i2.535Keywords:
gut microbiome, Microbial bioremediation, polystyrene degradation, Tenebrio molitorAbstract
Polystyrene is a petroleum-based polymer widely used in single-use packaging and thermal insulation. Its high molecular weight and structural stability make it highly resistant to degradation, leading to persistent environmental accumulation. Recent studies have highlighted the potential of insect gut microbiota, particularly from mealworms, to biodegrade plastic waste. In this study, gut extracts were inoculated into Nutrient Broth (NB) and Potato Dextrose Broth (PDB), each supplemented with polystyrene as emulsion, powder, or film. NB showed the highest reduction in polystyrene weight (0.93%) by day 28, compared to PDB (0.73%). Total plate count and yeast-mold count analyses revealed microbial proliferation in both media, with NB exhibiting a higher final bacterial count (7.562 log cfu/mL) than PDB (6.510 log cfu/mL), while fungal counts were comparable. Scanning Electron Microscopy confirmed structural damage to polystyrene films, including surface roughening and micro-pitting, especially in NB. These findings indicate that media composition significantly influences microbial growth and degradation efficiency. NB appears to favor bacterial communities with enhanced plastic-degrading capabilities, demonstrating superior biodegradation potential in liquid culture.
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Auta, H. S., Emenike, C. U. and Fauziah, S. H. 2017. Screening of Bacillus strains isolated from mangrove ecosystems in Peninsular Malaysia for microplastic degradation. Environmental Pollution 231(September): 1552–1559.
Calmont, B. and Soldati, F. 2008. Ecologie et biologie de Tenebrio opacus Duftschmid, 1812 Distribution et détermination des espèces françaises du genre Tenebrio linnaeus , 1758. R.a.R.E XVII (3): 81–87.
Ding, M. Q., Ding, J., Yang, S. S., Ren, X. R., Shi, S. N., Zhang, L. Y., et al. 2024. Effects of plastic aging on biodegradation of polystyrene by Tenebrio molitor larvae: Insights into gut microbiome and bacterial metabolism. Science of the Total Environment 953(September): 176130.
Jiang, J., Xu, H., Cao, X., Liang, Y., Mo, A., Cao, X., et al. 2024. Soil-dwelling grub larvae of Protaetia brevitarsis biodegrade polystyrene: Responses of gut microbiome and host metabolism. Science of the Total Environment 934.
Liberto, E. A. D., Battaglia, G., Pellerito, R., Curcuruto, G., dan Dintcheva, N. T. (2024): Biodegradation of Polystyrene by Plastic-Eating Tenebrionidae Larvae.” Polymers, 16.
Lin, W., Yao, Y., Su, T., dan Wang, Z. (2024): Biodegradation of Polystyrene by Bacteria Isolated from the Yellow Mealworm (Tenebrio molitor) Gut. Journal of Environmental Chemical Engineering, 12, 112071.
Lokesh, P., R. Shobika, Soghra Nashath Omer, Madhavi Reddy, Panchamoorthy Saravanan, R. Rajeshkannan, V. Saravanan, and S. Venkatkumar. 2023. Bioremediation of Plastics by the Help of Microbial Tool: A Way for Control of Plastic Pollution. Sustainable Chemistry for the Environment 3 (February): 100027.
Park, J. W., Kim, M., Kim, S. Y., Bae, J. and Kim, T. J. 2023. Biodegradation of polystyrene by intestinal symbiotic bacteria isolated from mealworms, the larvae of Tenebrio molitor. Heliyon 9(6).
Quan, Z., Zhao, Z., Liu, Z., Wang, W., Yao, S., Liu, H., et al. 2023. Biodegradation of polystyrene microplastics by superworms (larve of Zophobas atratus): Gut microbiota transition, and putative metabolic ways. Chemosphere 343.
Robinson, W. H. 2005. Handbook of urban insects and arachnids. Urban Insects and Arachnids.
Tsochatzis, E. D., Berggreen, I. E., Nørgaard, J. V., Theodoridis, G. and Dalsgaard, T. K. 2021. Biodegradation of expanded polystyrene by mealworm larvae under different feeding strategies evaluated by metabolic profiling using GC-TOF-MS. Chemosphere 281.
Tsochatzis, E., Lopes, J. A., Gika, H. and Theodoridis, G. 2021. Polystyrene biodegradation by Tenebrio molitor larvae: Identification of generated substances using a gc-ms untargeted screening method. Polymers 23(1): 1–12.
Urbanek, A. K., Rybak, J., Wróbel, M., Leluk, K. and Mirończuk, A. M. 2020. A comprehensive assessment of microbiome diversity in Tenebrio molitor fed with polystyrene waste. Environmental Pollution 262.
Wang, Q., Chen, H., Gu, W., Wang, S. and Li, Y. 2024. Biodegradation of aged polyethylene (PE) and polystyrene (PS) microplastics by yellow mealworms (Tenebrio molitor larvae). Science of the Total Environment 927.
Wang, S., Yu, H., Li, W., Song, E., Zhao, Z., Xu, J., et al. 2024. Biodegradation of four polyolefin plastics in superworms (Larvae of Zophobas atratus) and effects on the gut microbiome. Journal of Hazardous Materials 477.
Wu, C., Ma, Y., Wang, D., Shan, Y., Song, X., Hu, H., et al. 2022. Integrated microbiology and metabolomics analysis reveal plastic mulch film residue affects soil microorganisms and their metabolic functions. Journal of Hazardous Materials 423(PB): 127258.
Yang, S. S., Ding, M. Q., He, L., Zhang, C. H., Li, Q. X., Xing, D. F., et al. 2020. Biodegradation of polypropylene by yellow mealworms (Tenebrio molitor) and superworms (Zophobas atratus) via gut-microbe-dependent depolymerization. Science of the Total Environment 756: 144087.
Zhang, T., Li, X., Rao, X., Peng, Y., Zhao, C., Xu, Y., et al. 2024. Biodegradation of polystyrene and polyethylene by Microbacterium esteraromaticum SW3 isolated from soil. Ecotoxicology and Environmental Safety 274.
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Copyright (c) 2025 Afandi Afandi, Azzania Fibriani, Popi Septiani
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