Expression of rice resistance gene OsNPR1 against bacterial leaf blight on black rice Cempo Ireng cultivar after salicylic acid treatment
DOI:
https://doi.org/10.36782/apjsafe.v9i1.78Keywords:
Xanthomonas oryzae, OsNPR1, Cempo Ireng, chlorophyllAbstract
Black rice is an alternative staple food better than white rice. It has lower carbs, but higher antocyanin compared to white rice. Nowadays, black rice consumption has increased, production needs to be increased to accomodate the demand. But, to our knowledge, there is lack of information about black rice resistance against biotic stresses, one of black rice cultivar is Cempo Ireng. This information needed for optimal Cempo Ireng cultivation. In this research, we determined chlorophyl content and analyzed OsNPR1 expression of three cultivars: Java14 (resistant control), Cempo Ireng, and IR64 (susceptible control), against Xanthomonas oryzae (Xoo). All of them were sprayed with salicylic acid (SA) before inoculated with Xoo. IR64 24h had the lowest chlorophyll content (0,576 ± 0,066 mg/g), meanwhile Java14 72h had the highest content (2,358 ± 1,301 mg/g). However, all the cultivars did not show any correlation between them and time after Xoo inoculation. OsNPR1 expression did not show any significant change in Java14 and Cempo Ireng after being inoculation. However, IR64 showed increasing OsNPR1 in 72h and did not change in 96h after inoculation. It indicated that OsNPR1 played important role in IR64 resistance against Xoo, but not in Cempo Ireng and Java14.
Downloads
References
Apridamayanti P, Pratiwi R, Purwestri YA, Tunjung WAS & Rumiyati. 2017. Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia. Indonesian Journal of Biotechnology. 22(1):49-54.
Gnanamanickam SS. 2009. Biological Control of Rice Diseases. Springer. USA, pp.27-28.
Harborne JB. 1984. Phytochemical Methods: A Guide to Modern Technique of Plant Analysis. Chapman & Hall. London.
Jiang G, Yin D, Shi Y, Zhou Z, Li C, Liu P, Jia Y, Wang Y, Liu Z, Yu M, et al. 2020. OsNPR3.3-dependent salicylic acid signaling is involved in recessive gene xa5-mediated immunity to rice bacterial blight. Scientific Reports. 10:1-14.
Kumar A, Gul MZ, Zeeshan A, Bimolata W, Qureshi IA, & Ghazi IA. 2013. Differential antioxidative responses of three different rice genotypes during bacterial blight infection. Australian Journal of Crop Sciences. 7(12):1893-1900.
Lee A, Cho K, Jang S, Rakwal R, Iwahashi H, Agrawal GK, Shim J, & Han O. 2004. Inverse correlation between jasmonic acid and salicylic acid during early wound response in
rice. Biochemical and Biophysical Research Communications. 318:734-738.
Luna E, Bruce TJA, Roberts MR, Flors V, & Ton
J. 2012. Next-generation systemic acquired resistance. Plant Physiol. 158:844-853.
Shanti ML, Shenoy VV, Devi GL, Kumar VM, Premalatha P, Kumar GN, Shashidhar HE, Zehr UB, & Freeman WH. 2010. Marker- assisted breeding for resistance to bacterial leaf blight in popular cultivar and parental lines of hybrid rice. Journal of Plant Pathology. 92(2):495-501.
Shi L, Li P, Wang W, Gao M, Wu Z, Song X, & Hu D. 2015. Antibacterial Activity and Mechanism of Action of Sulfone Derivatives Containing 1,3,4-Oxadiazole Moieties on Rice Bacterial Leaf Blight. Molecules. 20:11660-11675.
Shimono M, Koga H, Akagi A, Hayashi N, Goto S, Sawada M, Kurihara T, Matsishita A, Sugano S, Jiang C, et al. 2012. Rice WRKY45 plays important roles in fungal and bacterial disease resistance. Mol. Plant Pathol. 13(1):83–94.
Solekha R. 2016. Analisis Proteomik Padi Hitam (Oryza sativa L.) Setekah Infeksi Xanthomonas oryzae pv. oryzae. Thesis. Pp. 12-14, 36-37.
Sugano S, Jiang CJ, Miyazawa SI, Masumoto C, Yazawa K, Hayashi N, Shimono M, Nakayama A, Miyao M, & Takatsuji H. 2010. Role of OsNPR1 in rice defense program as revealed by genome wide expression analysis. Plant Mol Biol. 74:549–562.
Takatsuji H. 2014. Development of disease- resistant rice using regulatory components of induced disease resistance. Strategies for disease-resistant rice. 5(630):1-12.
Tao Z, Liu H, Qiu D, Zhou Y, Li X, Xu C, & Wang S. 2009. A pair of allelic WRKY genes play opposite roles in rice-bacteria interactions. Plant Physiol. 151:936–948.
Thanh TL, Thumanu K, Wongkaew S, Boonkerd N, Teaumroong N, Phansak P, & Buensanteai
N. 2017. Salicylic acid-induced accumulation of biochemical components associated with resistance against Xanthomonas oryzae pv. oryzae in rice. Journal of Plant Interactions. 12(1):108-120.
Urry LA, Cain ML, Wasserman SA, Minorsky PV, & Orr RB. 2021. Biology. 12th ed. Pearson Education. USA, p.424.
Xu J, Audenaert K, Hofte M, & de Vleesschauwer
D. 2013. Abscisic Acid Promotes Susceptibility to the Rice Leaf Blight Pathogen Xanthomonas oryzae pv oryzae by Suppressing Salicylic Acid-Mediated Defenses. PLoS ONE. 8(6):1-10.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Ikhsan Maulana, Triyaningsih, Tri Rini Nuringtyas, and Yekti Asih Purwestri
This work is licensed under a Creative Commons Attribution 4.0 International License.
